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A Flow Cytometry Protocol for Understanding Export Mechanisms

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Flow Cytometry Analysis of Cell-miRNA and EV-miRNA Signal in EVs Isolated from Calu6 Cells Transfected with Fluorophore-Conjugated EV-miRNA

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Biology

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JoVE Journal Biology

Flow Cytometry Analysis of Cell-miRNA and EV-miRNA Signal in EVs Isolated from Calu6 Cells Transfected with Fluorophore-Conjugated EV-miRNA

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Flow Cytometry Analysis of Cell-miRNA and EV-miRNA Signal in EVs Isolated from Calu6 Cells Transfected with Fluorophore-Conjugated EV-miRNA

A Flow Cytometry Protocol for Understanding Export Mechanisms

DOI:

10.3791/201250-v

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03:01 min

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October 06, 2023

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Humna Hasan, Andrea L. Kasinski²

¹Department of Biological Sciences,Purdue University, ²Purdue Institute for Cancer Research,Purdue University

Tags

Flow Cytometry Extracellular Vesicles EV-miRNA Cell-miRNA Fluorophore-conjugated MiRNA Calu6 Cells Nano-flow Cytometry Performance Beads Instrument Calibration Gating Sample Preparation Data Analysis

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Flow Cytometry Analysis of Cell-miRNA and EV-miRNA Signal in EVs Isolated from Calu6 Cells Transfected with Fluorophore-Conjugated EV-miRNA

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