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Journal
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Bioengineering
/
Fabrication Followed by 3D Encapsulation of Tumor Spheroids in PEG Hydrogels
/
Immunofluorescence Fixation, Staining, and Clearing of Encapsulated Spheroids for Confocal Microscopy
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Immunofluorescence Fixation, Staining, and Clearing of Encapsulated Spheroids for Confocal Microscopy
Please note that all translations are automatically generated.
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Immunofluorescence Fixation, Staining, and Clearing of Encapsulated Spheroids for Confocal Microscopy
Fabrication Followed by 3D Encapsulation of Tumor Spheroids in PEG Hydrogels
DOI:
10.3791/201261-v
•
03:35 min
•
September 22, 2023
•
Joseph Bruns
,
Shabnam Nejat
,
Allison Faber
,
Silviya P. Zustiak
1
Biomedical Engineering Department
,
Saint Louis University
Tags
Immunofluorescence
Fixation
Staining
Clearing
Encapsulated Spheroids
Confocal Microscopy
Hydrogel
PBS
Fixative
Primary Antibodies
Nestin
SOX2
Formamide
Z-stack
Maximum Projection
DAPI
Stem Cell Marker
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