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Journal
/
Immunology and Infection
/
Development of pHluorin2-Based Sensor for Glycosomal pH in
Trypanosoma brucei
/
生きている
トリパノソーマ・ブルーセイ
のpHluorin2蛍光を測定するためのフローサイトメトリー用サンプル調製
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Sample Preparation for Flow Cytometry to Measure pHluorin2 Fluorescence in Living
Trypanosoma brucei
Please note that all translations are automatically generated.
Click here for the English version.
生きている
トリパノソーマ・ブルーセイ
のpHluorin2蛍光を測定するためのフローサイトメトリー用サンプル調製
Development of pHluorin2-Based Sensor for Glycosomal pH in
Trypanosoma brucei
DOI:
10.3791/201368-v
•
00:54 min
•
January 19, 2024
•
Daniel Call*
1
,
Sabrina S. Pizarro*
2,3
,
Erica Tovey
,
Emily Knight
3
,
Carrie Baumgardner
4
,
Kenneth A. Christensen
,
James C. Morris
1
Department Chemistry and Biochemistry
,
Brigham Young University
,
2
Eukaryotic Pathogens Innovation Center
,
Clemson University
,
3
Department of Genetics and Biochemistry
,
Clemson University
,
4
Department of Physics and Astronomy
,
Clemson University
Tags
Flow Cytometry
PHluorin2
Trypanosoma Brucei
Sample Preparation
Tetracycline
Doxycycline
Cell Culture
Centrifugation
PBS
Glucose
Propidium Iodide
Thiazole Red
Live/dead Staining
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check_url/201368?article_type=v
Measuring Dynamic Glycosomal pH Changes in Living
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check_url/201368?article_type=v
生きている
トリパノソーマ・ブルーセイ
のpHluorin2蛍光を測定するためのフローサイトメトリー用サンプル調製
check_url/201368?article_type=v
生きている
トリパノソーマ・ブルーセイ
のグリコソームの相対pHを測定するためのフローサイトメトリー手順
check_url/201368?article_type=v
生きた
トリパノソーマ・ブルーセイ
のグリコソームの相対pHを測定するためのフローサイトメトリーデータの解析
Read Article
