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Journal
/
Immunology and Infection
/
Development of pHluorin2-Based Sensor for Glycosomal pH in
Trypanosoma brucei
/
Canlı
Trypanosoma brucei'de
pHluorin2 Floresansını Ölçmek için Akış Sitometrisi için Numune Hazırlama
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Sample Preparation for Flow Cytometry to Measure pHluorin2 Fluorescence in Living
Trypanosoma brucei
Please note that all translations are automatically generated.
Click here for the English version.
Canlı
Trypanosoma brucei'de
pHluorin2 Floresansını Ölçmek için Akış Sitometrisi için Numune Hazırlama
Development of pHluorin2-Based Sensor for Glycosomal pH in
Trypanosoma brucei
DOI:
10.3791/201368-v
•
00:54 min
•
January 19, 2024
•
Daniel Call*
1
,
Sabrina S. Pizarro*
2,3
,
Erica Tovey
,
Emily Knight
3
,
Carrie Baumgardner
4
,
Kenneth A. Christensen
,
James C. Morris
1
Department Chemistry and Biochemistry
,
Brigham Young University
,
2
Eukaryotic Pathogens Innovation Center
,
Clemson University
,
3
Department of Genetics and Biochemistry
,
Clemson University
,
4
Department of Physics and Astronomy
,
Clemson University
Tags
Flow Cytometry
PHluorin2
Trypanosoma Brucei
Sample Preparation
Tetracycline
Doxycycline
Cell Culture
Centrifugation
PBS
Glucose
Propidium Iodide
Thiazole Red
Live/dead Staining
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