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Journal
/
Immunology and Infection
/
Development of pHluorin2-Based Sensor for Glycosomal pH in
Trypanosoma brucei
/
إجراء قياس التدفق الخلوي لقياس الرقم الهيدروجيني النسبي للجليكوسوم في
المثقبيات الحية بروسي
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Flow Cytometry Procedure to Measure Relative pH of the Glycosome in Living
Trypanosoma brucei
Please note that all translations are automatically generated.
Click here for the English version.
إجراء قياس التدفق الخلوي لقياس الرقم الهيدروجيني النسبي للجليكوسوم في
المثقبيات الحية بروسي
Development of pHluorin2-Based Sensor for Glycosomal pH in
Trypanosoma brucei
DOI:
10.3791/201369-v
•
02:15 min
•
January 19, 2024
•
Daniel Call*
1
,
Sabrina S. Pizarro*
2,3
,
Erica Tovey
,
Emily Knight
3
,
Carrie Baumgardner
4
,
Kenneth A. Christensen
,
James C. Morris
1
Department Chemistry and Biochemistry
,
Brigham Young University
,
2
Eukaryotic Pathogens Innovation Center
,
Clemson University
,
3
Department of Genetics and Biochemistry
,
Clemson University
,
4
Department of Physics and Astronomy
,
Clemson University
Tags
Flow Cytometry
Glycosome
PH
Trypanosoma Brucei
PHluorin
Propidium Iodide
Thiazole Red
Forward Scatter
Side Scatter
Relative Fluorescence Intensity
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Measuring Dynamic Glycosomal pH Changes in Living
Trypanosoma brucei
تحضير العينة لقياس التدفق الخلوي لقياس مضان pHluorin2 في
المثقبيات الحية brucei
إجراء قياس التدفق الخلوي لقياس الرقم الهيدروجيني النسبي للجليكوسوم في
المثقبيات الحية بروسي
تحليل بيانات قياس التدفق الخلوي لقياس الرقم الهيدروجيني النسبي للجليكوسوم في
المثقبيات الحية بروسي
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