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Journal
/
Immunology and Infection
/
Development of pHluorin2-Based Sensor for Glycosomal pH in
Trypanosoma brucei
/
Procedura di citometria a flusso per misurare il pH relativo del glicosoma in
Trypanosoma brucei vivente
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Flow Cytometry Procedure to Measure Relative pH of the Glycosome in Living
Trypanosoma brucei
Please note that all translations are automatically generated.
Click here for the English version.
Procedura di citometria a flusso per misurare il pH relativo del glicosoma in
Trypanosoma brucei vivente
Development of pHluorin2-Based Sensor for Glycosomal pH in
Trypanosoma brucei
DOI:
10.3791/201369-v
•
02:15 min
•
January 19, 2024
•
Daniel Call*
1
,
Sabrina S. Pizarro*
2,3
,
Erica Tovey
,
Emily Knight
3
,
Carrie Baumgardner
4
,
Kenneth A. Christensen
,
James C. Morris
1
Department Chemistry and Biochemistry
,
Brigham Young University
,
2
Eukaryotic Pathogens Innovation Center
,
Clemson University
,
3
Department of Genetics and Biochemistry
,
Clemson University
,
4
Department of Physics and Astronomy
,
Clemson University
Tags
Keywords: Flow Cytometry
Glycosome
PH
Trypanosoma Brucei
PHluorin
Propidium Iodide
Thiazole Red
Forward Scatter
Side Scatter
Relative Fluorescence Intensity
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