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AAV Isolation by Double Iodixanol Density Gradient Centrifugation
/
Dobbelt iodixanolcentrifugering til oprensning af adeno-associeret virus til in vivo-undersøgelser
JoVE Journal
Medicine
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JoVE Journal
Medicine
Double Iodixanol Centrifugation to Purify Adeno-Associated Virus for In Vivo Studies
Dobbelt iodixanolcentrifugering til oprensning af adeno-associeret virus til in vivo-undersøgelser
AAV Isolation by Double Iodixanol Density Gradient Centrifugation
DOI:
10.3791/201469-v
•
02:35 min
•
February 09, 2024
•
Kathryn K. Harris
,
Oleksandr Kondratov
,
Sergei Zolotukhin
1
Department of Pediatrics
,
University of Florida
Tags
Keywords: Adeno-associated Virus
AAV
Iodixanol
Ultracentrifugation
Purification
In Vivo Studies
Virus Clarification
Density Gradient
Centrifugation
Peristaltic Pump
Virus Fraction
15% Iodixanol
25% Iodixanol
40% Iodixanol
60% Iodixanol
Needle Aspiration
Virus Dilution
Lysis Buffer
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check_url/201469?article_type=v
Suspension kultur: produktion og oprensning af adeno-associeret virus ved iodixanol densitetsgradient centrifugering til in vivo applikationer
check_url/201469?article_type=v
Dobbelt iodixanolcentrifugering til oprensning af adeno-associeret virus til in vivo-undersøgelser
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