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Medicine
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AAV Isolation by Double Iodixanol Density Gradient Centrifugation
/
Centrifugação Dupla de Iodixanol para Purificar o Vírus Adenoassociado para Estudos In Vivo
JoVE Journal
Medicine
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JoVE Journal
Medicine
Double Iodixanol Centrifugation to Purify Adeno-Associated Virus for In Vivo Studies
Centrifugação Dupla de Iodixanol para Purificar o Vírus Adenoassociado para Estudos In Vivo
AAV Isolation by Double Iodixanol Density Gradient Centrifugation
DOI:
10.3791/201469-v
•
02:35 min
•
February 09, 2024
•
Kathryn K. Harris
,
Oleksandr Kondratov
,
Sergei Zolotukhin
1
Department of Pediatrics
,
University of Florida
Tags
Adeno-associated Virus
AAV Purification
Iodixanol Gradient
Ultracentrifugation
In Vivo Studies
Clarified Virus
15% Iodixanol
25% Iodixanol
40% Iodixanol
60% Iodixanol
Type 70 TI Rotor
AAV Lysis Buffer
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