JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biochemistry
/
Assessment of Hsp70 Chaperone Activity Using
Escherichia coli
dnaK756 Cells
/
Transformation of
E. coli
Cells for a Complementation Assay to Assess Hsp70 Chaperone Activity
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biochemistry
Transformation of
E. coli
Cells for a Complementation Assay to Assess Hsp70 Chaperone Activity
Transformation of
E. coli
Cells for a Complementation Assay to Assess Hsp70 Chaperone Activity
Assessment of Hsp70 Chaperone Activity Using
Escherichia coli
dnaK756 Cells
DOI:
10.3791/201542-v
•
03:31 min
•
March 08, 2024
•
Hloniphani Rachel Ncube
,
Unarine Dali
,
Tendamudzimu Harmfree Dongola
,
Addmore Shonhai
1
Department of Biochemistry & Microbiology
,
University of Venda
Tags
Keywords: E. Coli
DnaK756
Complementation Assay
Hsp70 Chaperone Activity
Heat Shock
Plasmid DNA
Transformation
Agar Plates
Antibiotic Selection
Serial Dilutions
Permissive And Non-permissive Growth Temperatures
Recombinant Protein Expression
KPf
KPf-V436F
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/201542?article_type=v
Author Spotlight: Exploring Heat Shock Proteins in Malaria and Tuberculosis Infections
check_url/201542?article_type=v
Transformation of
E. coli
Cells for a Complementation Assay to Assess Hsp70 Chaperone Activity
check_url/201542?article_type=v
Analysis of Hsp70 Chaperone Activity Using SDS PAGE and Western Blot Analyses of Transformed
E. coli
Protein
Read Article
