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Journal
/
Biochemistry
/
Assessment of Hsp70 Chaperone Activity Using
Escherichia coli
dnaK756 Cells
/
Трансформация клеток
E. coli
для анализа комплементации для оценки активности шаперона Hsp70
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Transformation of
E. coli
Cells for a Complementation Assay to Assess Hsp70 Chaperone Activity
Please note that all translations are automatically generated.
Click here for the English version.
Трансформация клеток
E. coli
для анализа комплементации для оценки активности шаперона Hsp70
Assessment of Hsp70 Chaperone Activity Using
Escherichia coli
dnaK756 Cells
DOI:
10.3791/201542-v
•
03:31 min
•
March 08, 2024
•
Hloniphani Rachel Ncube
,
Unarine Dali
,
Tendamudzimu Harmfree Dongola
,
Addmore Shonhai
1
Department of Biochemistry & Microbiology
,
University of Venda
Tags
Keywords: E. Coli
DnaK756
Complementation Assay
Hsp70 Chaperone Activity
Heat Shock
Plasmid DNA
Transformation
Agar Plates
Antibiotic Selection
Serial Dilutions
Permissive And Non-permissive Growth Temperatures
Recombinant Protein Expression
KPf
KPf-V436F
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