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Journal
/
Biochemistry
/
Assessment of Hsp70 Chaperone Activity Using
Escherichia coli
dnaK756 Cells
/
Анализ активности шаперона Hsp70 с использованием SDS PAGE и вестерн-блоттинг-анализа трансформированного белка
E. coli
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Analysis of Hsp70 Chaperone Activity Using SDS PAGE and Western Blot Analyses of Transformed
E. coli
Protein
Please note that all translations are automatically generated.
Click here for the English version.
Анализ активности шаперона Hsp70 с использованием SDS PAGE и вестерн-блоттинг-анализа трансформированного белка
E. coli
Assessment of Hsp70 Chaperone Activity Using
Escherichia coli
dnaK756 Cells
DOI:
10.3791/201543-v
•
02:00 min
•
March 08, 2024
•
Hloniphani Rachel Ncube
,
Unarine Dali
,
Tendamudzimu Harmfree Dongola
,
Addmore Shonhai
1
Department of Biochemistry & Microbiology
,
University of Venda
Tags
Keywords: Hsp70 Chaperone
SDS-PAGE
Western Blot
Transformed E. Coli
Protein Analysis
Laemmli SDS Loading Buffer
Electrophoresis
Coomassie Staining
Destaining
Protein Transfer
Nitrocellulose Membrane
Antibody Incubation
TBS-Tween Buffer
Secondary Antibody
Chemiluminescence Detection
Gel Imaging
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