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Journal
/
Biology
/
Identifying Drugs that Affect Polymerization of Bacterial Cytoskeletal Proteins
/
Målretting av GFP-merkede bakterielle cytoskjelettproteiner uttrykt i
Schizosaccharomyces pombe
og mikroskopisk avbildning av polymerene
JoVE Journal
Biology
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JoVE Journal
Biology
Targeting GFP-Tagged Bacterial Cytoskeletal Proteins Expressed in
Schizosaccharomyces pombe
and Microscopic Imaging of the Polymers
Målretting av GFP-merkede bakterielle cytoskjelettproteiner uttrykt i
Schizosaccharomyces pombe
og mikroskopisk avbildning av polymerene
Identifying Drugs that Affect Polymerization of Bacterial Cytoskeletal Proteins
DOI:
10.3791/201596-v
•
04:40 min
•
April 26, 2024
•
Sakshi Mahesh Poddar
2
,
Srijita Roy
2
,
Ajay Kumar Sharma
2
,
Ramanujam Srinivasan
2
1
School of Biological Sciences
,
National Institute of Science Education and Research
,
2
Homi Bhabha National Institutes
Tags
GFP-tagged
Bacterial Cytoskeletal Proteins
Schizosaccharomyces Pombe
Microscopic Imaging
PolymersEMM (Edinburgh Minimal Medium)
Concanavalin A
Epifluorescence Microscopy
96-well Plate
Adaptive Auto-focus
DIC (Differential Interference Contrast)
Fluorescence Imaging
Z-stack
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