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Single-Molecule Imaging of Nuclear Transport
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JoVE Journal Biology
Single-Molecule Imaging of Nuclear Transport

Single-Molecule Imaging of Nuclear Transport

DOI:
10.3791/2040-v

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12:13 min

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June 09, 2010

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, , ,
1Department of Biology,Bowling Green State University, 2Center for Photochemical Sciences,Bowling Green State University

Chapters

  • 00:00Title
  • 00:41Introduction
  • 01:20Preparation of Essential Proteins for Nuclear Transport Experiment
  • 06:10Preparation of the Cell System for Single Molecule Experiment
  • 08:52Single Molecule Microscopy
  • 10:22Representative Single Molecule Imaging
  • 11:39Conclusion

Summary

Automatic Translation

Automatic Translation

Single molecule microscopy approch provided novel insights into nuclear transport.

Tags

Single-molecule ImagingNuclear TransportSingle Molecule Fluorescence MicroscopyBiological ReactionsMolecular InteractionsHigh Temporal ResolutionHigh Spatial ResolutionNarrow-field Epifluorescence MicroscopyNucleocytoplasmic TransportSingle Molecule ApproachKineticsNuclear Transport TimeEfficiencySignal-dependent Cargo MoleculesSignal-independent Cargo MoleculesImproved Spatiotemporal ResolutionTransient Active TransportPassive Diffusion EventsNuclear Pore Complexes (NPC)Semi-intact CellsBinding EventsTrafficking Events

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