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质粒DNA重组流感病毒的一代
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Immunology and Infection
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JoVE Journal Immunology and Infection
Generation of Recombinant Influenza Virus from Plasmid DNA
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质粒DNA重组流感病毒的一代

DOI:
10.3791/2057-v

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11:31 min

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August 03, 2010

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1Department of Microbiology and Immunology,University of Rochester School of Medicine and Dentistry, 2Departments of Microbiology and Medicine, and Global Health and Emerging Pathogens Institute,Mount Sinai School of Medicine

Chapters

  • 00:04Title
  • 01:00Introduction
  • 01:29Influenza Virus Rescue Transfection
  • 04:54Infecting MDCK Cells & TC Harvest
  • 06:14Egg Infection & Allonotic Fluid Harvest
  • 07:55HA Assay
  • 08:55Confirmation of Rescue
  • 10:19Conclusion

Summary

Automatic Translation

Automatic Translation

质粒DNA的A型流感病毒的救援是一个基本的和必要的实验技术，使流感的研究人员，以产生重组病毒在流感病毒的生物学特性研究的多个方面，被视为潜在的载体或疫苗使用。

Tags

Recombinant Influenza VirusPlasmid DNAReverse GeneticsInfluenza ResearchInfectious VirusesViral ProteinsMutationsViral ReplicationPathogenesisViral VectorsForeign Proteins

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