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Chapters
- 00:00Title
- 01:15Introduction
- 01:53Overview of the Integrated Microscope System
- 03:26Preparation for AFM
- 06:37Mechanical Stimulation of the Live Cell
- 07:43Representative Results
- 08:48Conclusion
This paper aims to instruct the reader in the operation of an integrated atomic force-optical imaging microscope for mechanical stimulation of live cells in culture. A step-by-step protocol is presented. A representative data set that shows live cell response to mechanical stimulation is presented.
Tags
Live Cell ResponseMechanical StimulationIntegrated Optical MicroscopyAtomic Force MicroscopySub-cellular LevelSpatial ResolutionTemporal ResolutionTotal Internal Reflection Fluorescence MicroscopyFast-spinning Disk Confocal MicroscopyMolecular Dynamic StudiesAdherent Live CellsOptical ImagingReal-timeActin FilamentsFocal AdhesionsCellular StructureCell RestructuringDynamicsDetailed Protocol
