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In situ Protocol for Butterfly Pupal Wings Using Riboprobes
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In situ Protocol for Butterfly Pupal Wings Using Riboprobes

In situ Protocol for Butterfly Pupal Wings Using Riboprobes

DOI:
10.3791/208-v

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06:19 min

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May 28, 2007

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,
1Department of Biological Sciences,SUNY-University at Buffalo, 2Dept. Ecology and Evolutionary Biology,Yale University

Chapters

  • 00:09Collecting pre-pupae
  • 00:36Dissecting and fixing wings
  • 01:08Pre-hybridization
  • 02:27Making hybridization buffer
  • 03:09Hybridization
  • 03:59Applying antibody
  • 04:31Detection and development
  • 05:35Mounting
  • 05:59Result: patterns of Engrailed

Summary

Automatic Translation

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In order to examine gene expression in the pupal wing tissue of Bicyclus anynana, we present an optimized protocol for in situ hybridizations using riboprobes. We also provide guidelines for the further optimization of this protocol for use in pupal wings of other Lepidopteran species.

Tags

In Situ ProtocolButterfly Pupal WingsRiboprobesIn Situ HybridizationsGene ExpressionTranscriptionDevelopmental BiologySpatial PatternsTemporal PatternsAntibodiesProtein ProductsLarval Wing DiscsFragile Pupal Wings

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