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Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping
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Biology
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Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping

DOI:
10.3791/2122-v

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09:40 min

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August 26, 2010

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, , ,
1Schulich Faculty of Chemistry,The Technion, Israel Institute of Technology

Chapters

  • 00:00Title
  • 01:36Introduction
  • 02:12Overview of ESR Micro-imaging
  • 04:32ESR Micro-imaging Sample Preparation
  • 05:57ESR Micro-imaging Experiments
  • 07:50Representative Resuts for a 3D ESR Micro-imaging Experiment
  • 09:01Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a method for micron-scale three-dimensional imaging of oxygen concentration in the immediate environment of live cells by electron spin resonance microscopy.

Tags

Electron Spin ResonanceESR Micro-imagingOxygen MappingLive CellsOxygen LevelsMitochondrial FunctionsMetabolic FunctionsSignaling PathwaysMembrane PermeabilityDisease DifferentiationCellular MetabolismHigh-resolution Oxygen ImagingOptical FluorescencePhosphorescence MethodsExogenous Paramagnetic Probes

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