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Intraperitoneal injektion i vuxen Zebrafish
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Biology
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JoVE Journal Biology
Intraperitoneal Injection into Adult Zebrafish
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Intraperitoneal injektion i vuxen Zebrafish

DOI:
10.3791/2126-v

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09:19 min

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August 30, 2010

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, , ,
1Department of Organismal Biology and Anatomy,The University of Chicago, 2Committee on Molecular Metabolism and Nutrition,The University of Chicago, 3Department of Medicine,The University of Chicago

Chapters

  • 00:00Title
  • 00:54Introduction
  • 01:19Pre-injection Preparations
  • 05:34Cold-water Anesthesia, Injection, and Recovery
  • 07:56Blood Glucose Levels After 72 Hour Fast and Glucose Injection
  • 08:41Conclusion

Summary

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Vi visar intraperitoneal injektion i vuxen zebrafisk. Vi använder en 10 l NanoFil mikrospruta kontrolleras av en Micro4 controller och UltraMicroPump III. Denna demonstration innefattar användning av kallt vatten som bedövningsmedel.

Tags

Intraperitoneal InjectionAdult ZebrafishReagent AbsorptionMetabolic StudiesIP InjectionAbdominal CavityPelvic GirdleVeterinary MethodsZero MortalityBleeding At Injection SiteGentle HandlingFastingWeighingAnesthetizingRecoveryStress MinimizationSmall Injection Volume35G NeedleCortland Salt SolutionOsmotically BalancedFreshwater FishAeration Of GillsSurgical Plane Of Anesthesia

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