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Chapters
- 00:05Title
- 00:51Introduction
- 01:08Protein Overexpression
- 02:48Harvesting and Extracting the Cells
- 04:58Preparing the Membrane Fraction and Solubilizing Transmembrane Proteins
- 06:46Hexahistidine Tag Affinity Purification by IMAC
- 08:23Gel Filtration
- 09:44Representative Results from MexB Purification
- 10:23Conclusion
In this protocol we demonstrate the expression, solubilization, and purification of a recombinantly expressed membrane protein, MexB, as a soluble protein detergent complex. MexB is a multidrug resistance membrane transporter from the opportunistic bacterial pathogen Pseudomonas aeruginosa.
Tags
Membrane TransporterMexB Multidrug Resistance ProteinMultidrug ResistanceDrug Efflux PumpsPseudomonas AeruginosaTripartite ComplexesInner Membrane Proton-drug AntiporterOuter Membrane ChannelPeriplasmic Linker ProteinTransmembrane ProteinsProtein Detergent Complex (PDC)Detergent SolubilizationPurification
