/
/
An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:08Introduction
- 01:45The Syngeneic Model
- 03:43Cell Culture
- 04:34Pre-Surgery
- 05:48Surgery
- 07:27In vivo Imaging
- 09:20Representative in vivo and ex vivo Images
- 10:06Conclusion
To study in vivo tumor growth and tumor microenvironment, we used a syngeneic and orthotopic mouse model of ovarian cancer in immunocompetent animals. We transduced a mouse tumor cell line (MOV1) with Katushka fluorescent protein (MOV1KAT) and here we show its orthotopic implantation in ovary and in vivo imaging.
Tags
Orthotopic ModelSerous Ovarian CancerImmunocompetent MiceIn Vivo Tumor ImagingTumor Immune ResponsesTg-MISIIR-TAg Transgenic Mouse ModelSV40 Transforming RegionMullerian Inhibitory Substance Type II Receptor (MISIIR) Gene PromoterSyngeneic Allograft RecipientsPreclinical StudiesTumor Microenvironment
