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Journal
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Neuroscience
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成人北极黄鼠海马神经干细胞的生长和分化
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Growth and Differentiation of Adult Hippocampal Arctic Ground Squirrel Neural Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
成人北极黄鼠海马神经干细胞的生长和分化
DOI:
10.3791/2199-v
•
09:44 min
•
January 07, 2011
•
Kelly L. Drew
,
Rebecca C. McGee
,
Matthew S. Wells
,
Judith A. Kelleher-Andersson
1
Alaska Basic Neuroscience Program, Institute of Arctic Biology
,
University of Alaska at Fairbanks
,
2
Department Biochemistry
,
Hood College
,
3
Department of Cell Biology
,
Neuronascent, Inc.
,
4
Research and Development
,
Neuronascent, Inc.
Chapters
00:05
Title
01:03
Thawing, Seeding, and Expanding Arctic Ground Squirrel Neural Stem Cells (agsNSC)
01:05
Preparing poly-L-ornithine-coated Flasks
01:56
Thawing and Seeding agsNSC
03:39
Expanding agsNSC
04:11
Trypsinization of Cells
05:27
Inoculating Well Plates for Differentiation
07:25
Maintenance of Neurons
08:09
Results of Growth and Differentiation of agsNSC
09:29
Conclusion
Summary
Automatic Translation
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Automatic Translation
准备从成人非冬眠周岁北极地松鼠(AGS)海马神经干细胞。这些神经干细胞可以通过许多段落扩大,分化和保持了近50:50的神经元的神经胶质文化。
Tags
Growth
Differentiation
Adult Hippocampal
Arctic Ground Squirrel
Neural Stem Cells
Hibernation
Ischemic Injury
Brain
Oxygen-glucose Deprivation
Neurons
Proteins
Gene Targets
Therapies
Ischemic Indications
Cold Tolerance
Proliferation
Culture
TUJ1-positive Neurons
Toxic Chemicals
Neurogenesis
MEK/ERK Signaling Pathway
PI3K/Akt Signaling Pathway
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