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成人北极黄鼠海马神经干细胞的生长和分化
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Growth and Differentiation of Adult Hippocampal Arctic Ground Squirrel Neural Stem Cells
DOI:

09:44 min

January 07, 2011

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Chapters

  • 00:05Title
  • 01:03Thawing, Seeding, and Expanding Arctic Ground Squirrel Neural Stem Cells (agsNSC)
  • 01:05Preparing poly-L-ornithine-coated Flasks
  • 01:56Thawing and Seeding agsNSC
  • 03:39Expanding agsNSC
  • 04:11Trypsinization of Cells
  • 05:27Inoculating Well Plates for Differentiation
  • 07:25Maintenance of Neurons
  • 08:09Results of Growth and Differentiation of agsNSC
  • 09:29Conclusion

Summary

Automatic Translation

准备从成人非冬眠周岁北极地松鼠(AGS)海马神经干细胞。这些神经干细胞可以通过许多段落扩大,分化和保持了近50:50的神经元的神经胶质文化。

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