Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / In vivo Imaging of Intact Drosophila Larvae at…
JoVE Journal
Biology
Author Produced

This content is Open Access.

JoVE Journal Biology
In vivo Imaging of Intact Drosophila Larvae at Sub-cellular Resolution
 
Click here for the English version

In vivo Imaging of Intact Drosophila Larvae at Sub-cellular Resolution

Article DOI: 10.3791/2249-v • 17:51 min • September 10th, 2010
September 10th, 2010 •
*1,2, *1, 1,2, 1, 1, 1
1Junior Research Group Synaptic Plasticity, Hertie Institute for Clinical Brain Research, University of Tübingen, 2Graduate School of Cellular and Molecular Neuroscience, University of Tübingen
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

This protocol describes a reliable method for anesthetization and imaging of intact Drosophila melanogaster larvae. We have utilized the volatile anesthetic desflurane to allow for repetitive imaging at sub-cellular resolution and re-identification of structures for up to a few days1.

Tags

In Vivo Imaging Drosophila Larvae Sub-cellular Resolution Optical Imaging Genetically Encoded Fluorophores Genetic Tools Transgenic Animal Lines Anesthetize Larvae Desflurane Synaptic Populations High Degree Of Anesthetization Survival Rate Time-points Proteins Dynamics Glutamate Receptor Subunit GluR-IIA Endogenous Promoter Exon Trap Line FasII-GFP
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter