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Protein Crystallization for X-ray Crystallography
JoVE Journal
Biology
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JoVE Journal Biology
Protein Crystallization for X-ray Crystallography
DOI:

09:27 min

January 16, 2011

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Chapters

  • 00:00Title
  • 01:30Hanging Drop
  • 06:21Sitting Drop
  • 07:05Microbatch Procedure
  • 07:51Representative Results for Protein Crystallization
  • 08:50Conclusion

Summary

Automatic Translation

The 3-D structure of a molecule provides a unique understanding of how the molecule functions. The principal method for structure determination at near-atomic resolution is X-ray crystallography. Here, we demonstrate the current methods for obtaining three-dimensional crystals of any given macromolecule that are suitable for structure determination by X-ray crystallography.

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