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印记基因的DNA甲基化的测定拟南芥胚乳
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JoVE Journal Biology
Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm
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印记基因的DNA甲基化的测定拟南芥胚乳

DOI:
10.3791/2327-v

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09:23 min

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January 28, 2011

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, , , , ,
1Department of Biology,Saint Louis University

Chapters

  • 00:05Title
  • 01:11Genetic Crossing
  • 02:52Isolation of Endosperm Tissues in Arabidopsis
  • 03:46Bisulfite Treatment
  • 06:20PCR Amplification
  • 07:47Sequence Analysis and Determination of DNA Methylation Status: Representative Results
  • 09:00Conclusion

Summary

Automatic Translation

Automatic Translation

印记是在植物和哺乳动物繁殖的现象。 DNA甲基化起着重要作用的印迹机制。隔离胚乳和确定的印记基因的甲基化状态<em>拟南芥</em>可以是困难的。在这个协议中，我们介绍了如何隔离胚乳，并确定由亚硫酸氢钠测序的甲基化。

Tags

DNA MethylationImprinted GenesArabidopsis EndospermEpigenetic MechanismsDNA MethyltransferasesLoss Of DNA MethylationImprintingMaternal And Paternal AllelesReproduction DevelopmentFlowering PlantsDouble Fertilization EventMEDEA GeneFWA GeneDNA Methylation And DemethylationImprinting StatusMethylation PatternIsolate EndospermGenetic Cross

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