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Isolation of Mouse Salivary Gland Stem Cells
JoVE Journal
Biology
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JoVE Journal Biology
Isolation of Mouse Salivary Gland Stem Cells
DOI:

09:52 min

February 08, 2011

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Chapters

  • 00:05Title
  • 01:32Mechanical and Enzymatic Salivary Gland Tissue Digestion
  • 05:00Preparation of Salivary Gland Tissue for Plating
  • 07:32Plating Salivary Gland Cells
  • 08:39Examination of Salispheres and Proliferating Cells Using Phase-Contrast Microscopy and Immunostaining
  • 09:34Conclusion

Summary

Automatic Translation

An optimized protocol for the isolation of stem cells from the mouse salivary gland is described. The method employs enzymatic and mechanical digestion, and permits isolation of salispheres containing cells with characteristics of stem cells.

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