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高效率转导肝癌细胞的重组腺相关病毒血清型3载体

JoVE Journal
Medicine

This content is Free Access.

JoVE Journal Medicine

High-Efficiency Transduction of Liver Cancer Cells by Recombinant Adeno-Associated Virus Serotype 3 Vectors

Please note that all translations are automatically generated. Click here for the English version.

高效率转导肝癌细胞的重组腺相关病毒血清型3载体

DOI:

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19:02 min

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March 22, 2011

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Chen Ling, Yuan Lu, Binbin Cheng, Katherine E. McGoogan, Samantha W.Y. Gee, Wenqin Ma, Baozheng Li, George V. Aslanidi, Arun Srivastava

¹Department of Pediatrics, Division of Cellular and Molecular Therapy,University of Florida

Chapters

00:05Title
01:33Packaging of Recombinant Adeno-associated Virus R=Serotype 3 (rAAV3) Vectors
08:29Determination of rAAV3 Vector Titers
15:11rAAV3 Vector-mediated Transduction and Transgene Expression in Human Liver Cancer Cells
16:55A Representative Quantitative DNA Slot-blot and Representative Transduction of Hepatoblastoma Cells with rAAV3
18:32Conclusion

Summary

Automatic Translation

在这篇文章中，我们描述了鉴定的腺相关病毒血清型3（AAV3）针对人肝癌细胞的最有效的载体。

Tags

Recombinant Adeno-associated Virus Serotype 3 Vectors Liver Cancer Cells Gene Therapy AAV2 AAV Serotypes Human Liver Cancer Hepatoblastoma Hepatocellular Carcinoma Cellular Co-receptor High-efficiency Transduction Reporter Gene Potential Gene Therapy

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