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Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
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Chapters
- 00:05Title
- 00:58Introduction
- 01:27Prepare the PCB Electrodes
- 02:46Prepare the Microfluidic Channels
- 05:04Assemble the Microfluidic Channels onto the PCB Electrodes
- 05:53Sort and Concentrate Beads and Cells Using DEP
- 07:07Separate Beads and Cells Using DEP Actuation
- 09:25Conclusion
Dielectrophoresis (DEP) is an effective method to manipulate cells. Printed circuit boards (PCB) can provide inexpensive, reusable and effective electrodes for contact-free cell manipulation within microfluidic devices. By combining PDMS-based microfluidic channels with coverslips on PCBs, we demonstrate bead and cell manipulation and separation within multichannel microfluidic devices.
