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Intravital Microscopy of the Inguinal Lymph Node
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Intravital Microscopy of the Inguinal Lymph Node
DOI:

07:34 min

April 04, 2011

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Chapters

  • 00:05Title
  • 01:30Animal Preparation
  • 01:54Surgical Procedure
  • 03:54Imaging and Vessel Analysis
  • 05:57Evidence of LN and Vasculature Viability During Intravital Microscopic Evaluation
  • 07:00Conclusion

Summary

Automatic Translation

A technique for performing intravital microscopy of the inguinal lymph node (LN) is outlined. Such technique allows for real-time, in vivo study of the lymph node microvasculature and structure both during homeostasis and infection. This technique can be adapted to cell trafficking studies and to other lymph node sites.

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