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改性膜联蛋白V /碘化丙啶检测细胞凋亡细胞死亡的准确评估
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Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death
Please note that all translations are automatically generated.
Click here for the English version.
改性膜联蛋白V /碘化丙啶检测细胞凋亡细胞死亡的准确评估
DOI:
10.3791/2597-v
•
07:34 min
•
April 24, 2011
•
Aja M. Rieger
,
Kimberly L. Nelson
,
Jeffrey D. Konowalchuk
,
Daniel R. Barreda
2
1
Department of Biological Sciences
,
University of Alberta
,
2
Department of Agriculture, Food and Nutrition Sciences
,
University of Alberta
Chapters
00:05
Title
01:34
Cell Preparation
02:14
Application of Annexin V/PI Stain
04:26
RNase-treated Cells and Cell Lines Exhibit Fewer False Positive Staining Results
06:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
为评估细胞死亡的一种准确的方法是描述。该协议改进传统的膜联蛋白V /碘化丙碘化物（PI）的协议，这显示多达40％的假阳性的细胞株和原代细胞的动物模型的广泛事件。
Tags
Annexin V/Propidium Iodide Apoptosis Assay
Cell Death Studies
Flow Cytometry-based Methods
Plasma Membrane Integrity
Permeability
Cell Viability
Nuclear Stains
Late Apoptotic Cells
Necrotic Cells
False Positive Events
RNA Staining
Cytoplasmic Compartment
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