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كله يشن التحليل المناعى للالجنينية الاطراف الأوعية الدموية الجلدية : نظام نموذجي لدراسة الأوعية الدموية المتفرعة التشكل في الأجنة
JoVE Journal
Biology
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JoVE Journal Biology
Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
DOI:

09:53 min

May 20, 2011

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Chapters

  • 00:05Title
  • 01:42Collecting Embryonic Limb Skins
  • 03:53Whole-mount Immunohistochemical Staining of Limb Skins
  • 07:14Mounting the Limb Skins on a Slide
  • 08:24Results of Whole-mount Confocal Immunofluorescence Microscopy
  • 09:29Conclusion

Summary

Automatic Translation

We introduce a whole-mount immunohistochemistry and laser scanning confocal microscopy with multiple labelling for analyzing intricate vascular network formation in mouse embryonic limb skin.

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