/
/
Alta Sensibilidade de Detecção de 5 hydroxymethylcytosine-in Balb / C Tecido Cerebral
This content is Free Access.
Chapters
- 00:05Title
- 01:23DNA Glucosylation and Control Reactions
- 02:39Restriction Enzyme Digestion
- 03:58PCR / qPCR DNA Analysis
- 05:47Representative Results for the High Sensitivity Detection of 5-hydroxymethylcytosine
- 06:40Conclusion
O EpiMark 5-HMC e 5-mC Kit de análise podem ser usados para analisar e quantificar 5-metilcitosina e 5-hydroxymethylcytosine dentro de um locus spe específicos. O kit distingue 5 de 5 mC-HMC pela adição de glicose para o grupo hidroxila de 5-HMC através de uma reação enzimática utilizando β-glicosiltransferase (T4-BGT). Quando 5-HMC ocorre no contexto de CCGG, esta modificação converte um site MspI clivável para um site não-clivável.
Tags
High Sensitivity5-hydroxymethylcytosineDNA HydroxymethylationEpigenetic ResearchMammalian DNACytosineCpG DinucleotidesTet Family Of EnzymesBiomarkerMouse Embryonic Stem CellsNeuronal CellsBisulfite Sequencing MethodsLiquid ChromatographyMass Spectrometry AnalysisThin Layer ChromatographyAntibodiesDot Blot AnalysisImmunofluorescencePrecipitation Of Hydroxymethylated DNASingle Base Resolution
