A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:04Plating the Stem Cells
- 02:02Transfecting the Stem Cells
- 03:24Performing FRAP or FLIP
- 06:10FRAP and FLIP Data Analysis
- 07:26FRAP and FLIP Results
- 08:52Conclusion
We describe photobleaching methods including Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Loss In Photobleaching (FLIP) to monitor chromatin protein dynamics in embryonic stem (ES) cells. Chromatin protein dynamics, which is considered to be one of the means to study chromatin plasticity, is enhanced in pluripotent cells.
