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Immunology and Infection

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非手术治疗的小鼠气管内滴入肺和肺淋巴结流式细胞仪分析

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Immunology and Infection

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JoVE Journal Immunology and Infection

Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry

Please note that all translations are automatically generated. Click here for the English version.

非手术治疗的小鼠气管内滴入肺和肺淋巴结流式细胞仪分析

DOI:

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08:01 min

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May 02, 2011

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Manira Rayamajhi, Elizabeth F. Redente, Tracy V. Condon, Mercedes Gonzalez-Juarrero, David W.H. Riches^2,4, Laurel L. Lenz⁴

¹Department of Immunology,University of Colorado School of Medicine, ²Division of Cell Biology, Department of Pediatrics,National Jewish Health , ³Department of Microbiology, Immunology, and Pathology,Colorado State University, ⁴Department of Immunology,National Jewish Health

Chapters

00:05Title
01:32Intratracheal Injection
03:11Harvesting and Processing Lung Draining Lymph Nodes and Lungs for Flow Cytometry
06:33Representative Flow Cytometric Analyses of Lung Draining Lymph Nodes After Intratracheal Injection
07:39Conclusion

Summary

Automatic Translation

我们说明进入肺部通过气管内麻醉小鼠的非手术治疗提供试验材料。这种方法允许肺暴露于细菌和病毒的病原体，细胞因子，抗体，珠子，化学品，染料。我们进一步描述采伐和肺和肺淋巴结（LDLNs），流式细胞仪检测处理。

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