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Journal
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Immunology and Infection
/
一个钙生物发光检测蚊虫的功能分析(
埃及伊蚊
)和刻度(
镰形扇头microplus
)G蛋白偶联受体
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
A Calcium Bioluminescence Assay for Functional Analysis of Mosquito (
Aedes aegypti
) and Tick (
Rhipicephalus microplus
) G Protein-coupled Receptors
Please note that all translations are automatically generated.
Click here for the English version.
一个钙生物发光检测蚊虫的功能分析(
埃及伊蚊
)和刻度(
镰形扇头microplus
)G蛋白偶联受体
DOI:
10.3791/2732-v
•
10:46 min
•
April 20, 2011
•
Hsiao-Ling Lu
,
Cymon N. Kersch
,
Suparna Taneja-Bageshwar
2
,
Patricia V. Pietrantonio
1
Department of Entomology
,
Texas A&M University (TAMU)
,
2
Department of Molecular and Cellular Medicine
,
Texas A&M University (TAMU)
Chapters
00:05
Title
01:40
Establishment of Stable Cell Lines
03:37
Selection of Clonal Cell Lines
05:40
Plate Preparation for the Calcium Bioluminescence Assay
07:20
Calcium Bioluminescence Assay: Instrument Operation and Data Analysis
08:45
Results: Calcium Bioluminescence Assay for Functional Analysis of G Protein-Coupled Receptors
10:22
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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italiano (Italian)
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Automatic Translation
该协议提供克隆细胞系的选择和钙生物发光检测,以分析其同源GPCRs的合成节肢动物神经肽的构效关系的说明。此法可用于受体deorphanization和构效关系研究合成的模拟设计和肽/药物导致发现。
Tags
Calcium Bioluminescence Assay
Functional Analysis
Mosquito
Aedes Aegypti
Tick
Rhipicephalus Microplus
G Protein-coupled Receptors
Arthropod Hormone Receptors
Pesticides
Kinin Receptors
Neuropeptides
Structure-activity Relationships
Heterologous Kinin Receptor-expressing Systems
Biostable Kinin Analogs
Diuretic Processes
Myotropic Processes
Digestive Processes
Southern Cattle Tick
Boophilus Microplus
Aedes Aegypti
CHO-K1 Cell Line
Calcium Levels
Peptide Application
Recombinant Cells
Aequorin Protein
Article
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