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从石蜡包埋材料的遗传和表观遗传分析的DNA提取
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal
Biology
DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses
Please note that all translations are automatically generated.
Click here for the English version.
从石蜡包埋材料的遗传和表观遗传分析的DNA提取
DOI:
10.3791/2763-v
•
13:32 min
•
March 26, 2011
•
Larissa A. Pikor*
1,2
,
Katey S. S. Enfield*
1,2
,
Heryet Cameron
,
Wan L. Lam
2,4
1
Department of Integrative Oncology
,
BC Cancer Research Centre
,
2
Interdisciplinary Oncology Program
,
University of British Columbia – UBC
,
3
Photography/Video Production, Multi-Media Services
,
BC Cancer Agency
,
4
Department of Pathology and Laboratory Medicine
,
University of British Columbia – UBC
Chapters
00:05
Title
00:38
Paraffin Removal
01:40
Ethanol Rehydration
02:58
Tissue Digestion
04:09
DNA Clean Up
07:12
DNA Precipitation
10:40
DNA Quantification
Summary
Automatic Translation
English (Original)
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Automatic Translation
本视频演示了从福尔马林固定石蜡包埋的材料DNA提取的协议。这是一个多天的过程中,水化组织切片用二甲苯deparaffinized,乙醇和蛋白酶K处理,净化和隔离随后的特定基因或全基因组分析的DNA。
Tags
DNA Extraction
Paraffin Embedded Material
Genetic Analysis
Epigenetic Analysis
Chromosomal Rearrangements
Copy Number Gains And Losses
DNA Methylation
High-throughput Profiling Technologies
Microarrays
Sample Quality
Specimen Collection
Formalin-fixed And Paraffin Embedded (FFPE)
Hospital Archives
Extraction Method
Xylene Treatment
Ethanol Washes
Proteinase K
Lysis Buffer
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