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A Protocol for Collecting and Staining Hemocytes from the Yellow Fever Mosquito Aedes aegypti
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Chapters
- 00:05Title
- 01:27Preparation for Hemocyte Collection
- 04:05Hemocyte Collection
- 06:17Hemocyte Fixation and Staining
- 07:14Identifying and Counting Aedes aegypti Hemoctyes
- 08:00Conclusion
A simplified yet accurate method to collect and stain mosquito hemocytes is described. Our method combines the simplicity of perfusion with the accuracy of high injection techniques to isolate clean preparations of hemocytes in Aedes mosquitoes. This method facilitates studies requiring knowledge of the types of hemocytes and their abundance.
Tags
ProtocolCollectingStainingHemocytesYellow Fever MosquitoAedes AegyptiVectorsDisease-causing PathogensYellow Fever VirusMalaria ParasitesFilarial WormsInnate Immune SystemDefense LinesEpithelium-lined Mosquito MidgutHemolymphCirculating HemocytesEncapsulate And Engulf PathogensAdult EmergenceDecline With AgeCollect HemocytesMedically Important InsectsSmall Size MosquitoesLimited Volume Of HemolymphExpulsion MethodCut ProboscisVolume Displacement MethodSaline Injection
