Lentiviral-mediated Knockdown During <em>Ex Vivo</em> Erythropoiesis of Human Hematopoietic Stem Cells

Carmen G. Palii; Roya Pasha; Marjorie Brand

doi:10.3791/2813

Journal

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Biology

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Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells

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Biology

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JoVE Journal Biology

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells

DOI:

10.3791/2813-v

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14:22 min

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July 16, 2011

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Carmen G. Palii, Roya Pasha, Marjorie Brand²

¹The Sprott Center for Stem Cell Research, Regenerative Medicine Program,Ottawa Hospital Research Institute, ²Department of Cellular and Molecular Medicine,University of Ottawa

Chapters

00:05Title
02:17Ex vivo Erythropoiesis of Human Hematopoietic Stem/Progenitor Cells
06:40Lentiviral Mediated Knockdown During ex vivo Erythroid Differentiation
10:19Results of ex vivo Erythropoiesis of Human Hematopoietic Stem Cells
13:37Conclusion

Summary

Automatic Translation

An ex vivo protocol to generate mature human red blood cells from hematopoietic stem/progenitors is described. Additionally we describe an efficient lentiviral-delivery method to knockdown the transcription factor TAL1 in primary erythroid cells. The efficiency of lentivirus mediated gene delivery is demonstrated using GFP expressing viruses.