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Chapters
- 00:05Title
- 01:54Dissection of the Adult Zebrafish Kidney from an Unfixed Animal Sample
- 03:59Preparation and Dissection of the Adult Zebrafish Kidney from a Fixed Animal Sample
- 05:48Identification of an in situ Whole Adult Kidney and Whole Mount in situ Hybridizations of Whole Adult Kidney Gene Transcripts
- 07:00Conclusion
The zebrafish kidney is home to both renal and hematopoietic adult stem/progenitor cells, and represents an outstanding opportunity to study these cell types and their progeny in a vertebrate model organism. Here, we demonstrate a detailed dissection procedure that enables the researcher to identify and surgically remove the adult zebrafish kidney, which can be used for applications such as cell isolation, transplantation, and expression studies of kidney and/or blood cell populations.
Tags
DissectionAdult Zebrafish KidneyStem Cell BiologySignalsBehaviorFunctionHomeostasisDisease StatesRegenerative MedicineTherapeutic StrategiesOrgansIn VitroAnimal ModelVertebrate Cell BiologyEmbryonic DevelopmentAnatomySurgical Dissection TechniquesResearch OrganismAdult StudiesStem Cell PopulationsCancerHematopoiesisKidney Composition
