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Chapters
- 00:05Title
- 01:33Preparing the Micropipettes for Microiontophoresis
- 03:13Sample Set Up and Microiontophoresis
- 04:52Fluorescence Response in Arteriole Wall
- 05:27Conclusion
Microiontophoresis entails movement of ions from a micropipette in response to a difference in electrical potential between the inside and outside of the micropipette. Biologically active molecules are thereby delivered in proportion to electrical current. We illustrate acetylcholine microiontophoresis in conjunction with micromanipulation to study endothelium-dependent vasodilation in the microcirculation.
Tags
MicroiontophoresisMicromanipulationIntravital Fluorescence ImagingMicrocirculationSolute DeliverySynaptic TransmissionNeurotransmittersNeuropeptidesVasodilationVasoconstrictionVasomotor ResponsesMicrovesselsConcentrationEjection CurrentStimulus-response RelationshipsSolute ConcentrationIonizationMicropipette Tip
