Journal
/
Bioengineering
/
Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

DOI:
10.3791/2922-v

•

13:15 min

•

June 01, 2011

•

, , ,
1Department of Chemistry and Biochemistry,University Of California Santa Barbara, 2Department of Chemistry and Biochemistry, Program in BioMolecular Science and Engineering,University Of California Santa Barbara

Chapters

  • 00:05Title
  • 01:28Preparation of Chemically Modified Probe DNA
  • 02:48Sensor Preparation
  • 05:30Sensor Testing, DNA Detection
  • 07:57Sensor Testing, Antibody Detection
  • 09:47Sensor Testing, Small Molecule Detection
  • 11:24Representative Results obtained using Electrochemical-DNA Biosensors
  • 12:27Conclusion

Summary

Automatic Translation

Automatic Translation

"E-DNA" sensors, reagentless, electrochemical biosensors that perform well even when challenged directly in blood and other complex matrices, have been adapted to the detection of a wide range of nucleic acid, protein and small molecule analytes. Here we present a general procedure for the fabrication and use of such sensors.

Tags

Electrochemical-DNA BiosensorsReagentless DetectionNucleic AcidsProteinsSmall MoleculesBedside TestsRapid DetectionQuantitative DetectionClinical SamplesEnvironmental SamplesE-DNA BiosensorsTarget DNA Sequence DetectionPolymerase Chain Reaction MixtureHIV-specific Antibody DetectionDrug Cocaine Detection

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2922?article_type=v

On-chip Isotachophoresis for Separasjon av ioner og rensing av nukleinsyrer
check_url/2922?article_type=v

Optisk deteksjon av<em> E. coli</em> bakterier ved Mesoporous Silicon biosensorer
check_url/2922?article_type=v

Perle aggregering Analyser for karakterisering av Antatte celleadhesjonsmolekyler
check_url/2922?article_type=v

Design og fabrikasjon av en Elastomeric Enhet for Soft Modular Robots i minimal invasiv kirurgi
check_url/2922?article_type=v

Levering av proteiner, peptider eller celle-ugjennomtrengelig små molekyler inn i levende celler ved inkubasjon med den Endosomolytic Reagens dfTAT
check_url/2922?article_type=v

Fast Enzymatisk Behandling av proteiner for MS Detection med en Gjennomstrømning mikroreaktor
check_url/2922?article_type=v

Utvikling av en elektrokjemisk DNA Biosensor å oppdage en infeksjon patogen
check_url/2922?article_type=v

Visuell gjenkjenning av flere nukleinsyrer i kapillær arrayet
check_url/2922?article_type=v

Utviklingen av Microfluidic enheter å studere forlengelse evnen til tips voksende anlegget celler i svært små områder
check_url/2922?article_type=v

Fabrikasjon av Decellularized brusk-avledet Matrix stillaser

Read Article