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Investigating Outer Hair Cell Motility with a Combination of External Alternating Electrical Field Stimulation and High-speed Image Analysis
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Chapters
- 00:05Title
- 01:19Isolation of Outer Hair Cells
- 03:05Experimental Setup
- 05:50External Alternating Electrical Field Stimulation and Image Capture
- 06:54Representative Results for Outer Hair Cell Motility
- 08:57Conclusion
A reliable method to investigate outer hair cell (OHC) motile responses, including electromotility, slow motility and bending, is described. OHC motility is elicited by stimulation with an external alternating electrical field, and the method takes advantage of high-speed image recording, LED-based illumination, and last generation image analysis software.
Tags
Outer Hair Cell MotilityExternal Alternating Electrical Field StimulationHigh-speed Image AnalysisOHCsOrgan Of CortiAuditory OrganStereociliaSound Energy DetectionTransductionCochlear AmplificationElectromotilityFast MotilityMotor ProteinsOHC Plasma MembraneSlow MotilityCytoskeletal ReorganizationOHC BendingAsymmetric Distribution Of Motor ProteinsElectrical Field StimulationMechanical StimuliChemical Stimuli
