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1Temple University,Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology, 2Medical Research Service,Department of Veterans Affairs Hospital, 3Department of Neurobiology and Anatomy,Drexel University College of Medicine, 4Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology,Temple University School of Medicine
Chapters
- 00:05Title
- 01:19Preparation for Surgery
- 02:19Laminectomy and Surgical Exposure of the L5 Dorsal Root
- 03:21Rhizotomy/Dorsal Root Crush
- 04:10Image Acquisition and Post-op Procedures
- 04:51Repeated Imaging
- 05:32Axonal Regeneration Following Dorsal Root Crush
- 06:16Conclusion
Ein<em> In vivo</em> Bildgebungsprotokoll zu primären sensorischen Axone folgenden Spinalganglien crush-Monitor beschrieben. Die Verfahren nutzen wide-field Fluoreszenzmikroskopie und THY1-YFP transgenen Mäusen, und erlauben wiederholte Bildgebung Axonregeneration über 4 cm im PNS und Axon Wechselwirkungen mit der Oberfläche des ZNS.
Tags
Live ImagingDorsal Root AxonsRhizotomySpinal Root InjuriesChronic PainSensory LossRegenerationDorsal Root Entry Zone (DREZ)Molecular EventsCellular EventsNerve InjuryPostmortem AnalysesAxon RegenerationReal-time EventsLiving AnimalNeurons Serially In VivoOpticsMouse TransgenicsThy1-GFP Transgenic MiceFluorescent ColorsIn Vivo ImagingMuscleBrainPhysiological MechanismsStatic AnalysesSpinal Cord Imaging
