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Chapters
- 00:05Title
- 01:25Two-photon Activation of Caged Fluorescein-dextran
- 05:03Immunodetection of Uncaged Fluorescein-dextran
- 06:04Photoactivation and Immunodetection of Caged Fluorescein-dextran
- 07:11Conclusion
En metod beskrivs för att photoactivate enskilda celler innehållande en bur fluorescerande protein med två-foton-absorption från en Ti: Sapphire femtosecond laseroscillatorn. För ödet karta fotoaktiverad cell, immunohistokemi används. Denna teknik kan tillämpas på varje celltyp.
Tags
Single Cell Fate MappingZebrafishDevelopmental BiologyFate MappingLineage TracingUndifferentiated Precursor CellsPhotoactivatable ProteinsFluorescence SpectrumUV ExcitationCell Tracing ProbesLong-term Cell TrackingCaged Fluorescein-dextranEmbryo Model SystemsUncaging Fluorescein-dextranSpatial ResolutionPhotoactivationNear-infrared Laser PulsesTitanium Sapphire Femtosecond LaserTwo-photon Confocal Microscopes
