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Lensless Fluorescent Microscopy on a Chip
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Lensless Fluorescent Microscopy on a Chip
DOI:

11:23 min

August 17, 2011

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Chapters

  • 00:05Title
  • 01:29Design of the Lensless On-Chip Imaging Platform
  • 03:44Assembly of the Lensless On-Chip Microscopy Platform
  • 05:03Sample Preparation
  • 06:18Imaging
  • 08:02Lensless Fluorescent Microscopy Results
  • 10:48Conclusion

Summary

Automatic Translation

A lensless on-chip fluorescent microscopy platform is demonstrated that can image fluorescent objects over an ultra-wide field-of-view of e.g., >0.6-8 cm2 with <4μm resolution using a compressive sampling based decoding algorithm. Such a compact and wide-field fluorescent on-chip imaging modality could be valuable for high-throughput cytometry, rare-cell research and microarray-analysis.

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