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表达GFP -肌动蛋白的内皮细胞的肌动蛋白细胞骨架的研究
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin
DOI:

08:37 min

November 18, 2011

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Chapters

  • 00:05Title
  • 01:27Transfection of HUVEC with GFP-Actin
  • 03:25Set up: Live-cell Imaging Chamber and Stage Heater
  • 05:20Live-cell Imaging Microscope Data Acquisition and Analysis
  • 06:14Kymograph Analysis of HUVEC
  • 08:08Conclusion

Summary

Automatic Translation

显微成像可以表达GFP -肌动蛋白的内皮细胞骨架结构的动态变化特征。这种方法与使用固定标本的技术,在之前的相同细胞的肌动蛋白细胞骨架的时空变化提供了详细的评估,期间和之后,各种生理,药理,或炎症刺激。

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