Journal
/
Biology
/
Hsp104纯化，蛋白质Disaggregase
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Purification of Hsp104, a Protein Disaggregase
Please note that all translations are automatically generated. Click here for the English version.

Hsp104纯化，蛋白质Disaggregase

DOI:
10.3791/3190-v

•

07:17 min

•

September 30, 2011

•

, ,
1Department of Biochemistry and Biophysics,University of Pennsylvania

Chapters

  • 00:05Title
  • 01:07Cell Harvest and Lysis
  • 01:55Ni-affinity Purification of Hsp104
  • 03:16FPLC Purification of Hsp104
  • 05:14Representative Results for Hsp104 Purification and Assessment of Disaggregation Activity
  • 07:04Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们描述一个高度活跃Hsp104，一个六聚AAA +从酵母中的蛋白质，其中夫妇ATP水解蛋白分解净化的协议。该计划利用从亲和纯化His6标签的​​构造<em> E。大肠杆菌</em>其次，His6标签去除TEV蛋白酶，体积排阻色谱阴离子交换层析。

Tags

PurificationHsp104Protein DisaggregaseHexameric AAA+ ProteinATP HydrolysisProtein DisaggregationRenaturationDisordered AggregatesHeat ShockCross-beta Amyloid FibrilsPrionsPhenotypic VariationPreamyloid OligomersE. Coli Orthologue ClpBHsp104 OrthologuesAnimal CellsTherapeutic AgentNeurodegenerative DiseasesMisfolding Of ProteinsToxic Preamyloid OligomersAmyloid Fibrils

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/3190?article_type=v

无有机溶剂和醋酸，蛋白质与考马斯亮蓝G - 250凝胶染色
check_url/3190?article_type=v

纯化 M。 magneticum应变AMB - 1磁小体相关蛋白MamAΔ41
check_url/3190?article_type=v

表达，洗涤剂溶解，和一个膜转运，MexB多药耐药蛋白的纯化
check_url/3190?article_type=v

自动疏水层析蛋白质纯化用柱选择
check_url/3190?article_type=v

高活性酵母核糖体的色谱分离纯化
check_url/3190?article_type=v

由一个小型的双特异性亲和标签的推动正交蛋白质纯化
check_url/3190?article_type=v

鉴定蛋白质相互作用的合作伙伴使用串联亲和纯化
check_url/3190?article_type=v

侵入细胞DISC1蛋白种类的产生，纯化及表征
check_url/3190?article_type=v

蛋白质纯化方法测定结合亲和力微尺度热泳
check_url/3190?article_type=v

鉴定蛋白质 - 蛋白质相互作用的<em>果蝇</em>成人头由串联亲和纯化（TAP）

Read Article