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Imaging Leukocyte Adhesion to the Vascular Endothelium at High Intraluminal Pressure
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Chapters
- 00:05Title
- 01:11Mounting the Vessel
- 02:13Pressurizing the Vessel
- 04:19Perfusion with While Blood
- 05:18Representative Leukocyte Adhesion Results
- 06:01Conclusion
This is a method to visualise leukocyte adhesion to the endothelium in harvested pressurised vessels. The technique enables studying vascular adhesion under shear flow with differing intraluminal pressures up to 200 mmHg thus mimic-ing the pathophysiological conditions of high blood pressure.
Tags
Leukocyte AdhesionVascular EndotheliumHigh Intraluminal PressureHypertensionEndothelial DysfunctionInflammationAtherosclerosisChronic Kidney DiseaseStrokeHeart FailureAdhesion CascadeRollingTetheringAdherenceTransmigrationAdhesion MoleculesVCAM-1ICAM-1E-selectinCytokinesChemokinesReactive Oxygen SpeciesStatic Adhesion AssaysCell-to-cell AdhesionCell Adhesion MoleculesIn Vitro MethodsPressure On EndotheliumMonocyte AdhesionInflammatory MarkersPhysiological Flow Conditions
