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采用TaqMan从儿童凳诺如病毒的检测和Genogrouping一步法RT-PCR技术
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JoVE Journal Medicine
Detection and Genogrouping of Noroviruses from Children’s Stools By Taqman One-step RT-PCR
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采用TaqMan从儿童凳诺如病毒的检测和Genogrouping一步法RT-PCR技术

DOI:
10.3791/3232-v

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15:16 min

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July 22, 2012

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, , , , , , , , ,
1Laboratorio de Investigación y Desarrollo (LID),Universidad Peruana Cayetano Heredia, 2Bloomberg School of Public Health,Johns Hopkins University, 3Laboratorio de Diagnostico Molecular, Facultad de Medicina,University of Concepcion,Chile, 4University of California San Diego School of Medicine

Chapters

  • 00:05Title
  • 01:37In-house Silica-guanidinium Method
  • 02:02Preparation of Silica Suspension for RNA Extraction
  • 03:24Extraction with Guanidine & Silica
  • 06:08Commercial Extraction of Viral RNA: QIAamp Viral RNA Kit
  • 08:32Detection of NoV in Extracted RNA by One-step Real Time PCR with Specific Taqman Probes
  • 14:11Conclusion

Summary

Automatic Translation

Automatic Translation

一个一步法RT-PCR方法检测检测和孩子的大便，，利用引物和的TaqMan探针的开放阅读框（ORF1基因）的病毒ORF2交界地区，对诺沃克类病毒基因组中最保守的地区是特定genogroup诺沃克病毒分离鉴定描述。详述一个非商业化，具有成本效益的RNA提取方法。

Tags

NorovirusesGenogroupingTaqman One-step RT-PCRAcute GastroenteritisRNA VirusesGenetic DiversityNew StrainsGenogroupsGIGIIGenotypesSubtypesDiagnosisTreatmentRNA ExtractionQIAmp Viral RNA Commercial KitSilica Gel MembraneBuffersMicrospinDiarrheaPathogenesis

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