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Prøvepræparation, Imaging, og Analyse protokoller for knivsæg Scanning Mikroskopi
This content is Free Access.
1Department of Computer Science and Engineering,Texas A&M University, 2Beckman Institute for Advanced Science and Technology,University of Illinois, 3Department of Electrical and Computer Engineering,Kettering University, 43Scan, 5Department of Veterinary Integrative Biosciences,Texas A&M University
Chapters
- 00:05Title
- 01:27Specimen Preparation: Golgi-Cox Method
- 03:20Specimen Preparation: Nissl Method
- 04:39Specimen Preparation: India Ink Method
- 05:37KESM Setup and Imaging
- 06:38Image Processing, Data Visualization and Analysis
- 08:12Representative Results of KESM
- 10:00Conclusion
Den fulde proces fra hjernen prøvepræparation til seriel inddele billeddannelse ved hjælp af Knife-Edge Scanning Microscope, at data visualisering og analyse er beskrevet. Denne teknik er i øjeblikket anvendes til at erhverve mus hjernen data, men det gælder for andre organer, andre arter.
Tags
Specimen PreparationImagingAnalysis ProtocolsKnife-edge Scanning MicroscopyHigh-throughput MicroscopyHigh-resolution Microscopy3D MicroscopyNeuroanatomical DataSubmicrometer ResolutionWhole-brain-scale DataNeuronal NetworksVascular NetworksCell Body DistributionConnectomics ResearchMicrocirculation ResearchHemodynamic ResearchStereology ResearchFixingStainingEmbeddingKESM Configuration And SetupSectioning And Imaging With KESMImage ProcessingData PreparationData VisualizationData Analysis
