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MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells
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Biology
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JoVE Journal Biology
MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells

MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells

DOI:
10.3791/3305-v

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09:12 min

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December 21, 2011

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, , , ,
1Sigma-Aldrich

Chapters

  • 00:05Title
  • 01:40Transduction and Treatment of Target Cells
  • 04:04Deconvolution from a Polyclonal Cell Population
  • 06:16Target Identification and Validation
  • 07:25Representative Screening Results Identifying Genes Involved in the Cytotoxicity of A549 Cells when Exposed to TRAIL and Paclitaxel
  • 08:51Conclusion

Summary

Automatic Translation

Automatic Translation

Here we use a human LentiPlex pooled library and traditional sequencing methods to identify gene targets promoting cell survival. We demonstrate how to set up and deconvolute a LentiPlex screen and validate the results.

Tags

MISSION LentiPlexPooled ShRNA LibraryScreeningMammalian CellsRNA InterferenceGene Expression RegulationKnockdownLoss Of Gene Function StudiesSmall Interfering RNAsSiRNAsShort-hairpin RNAsShRNAsPlasmidsLentiviral ParticlesLentiviral ShRNAsIntroduction Into Cell TypesLong Term Gene KnockdownSelectionHigh-throughput Loss Of Function ScreensLentiPlex Pooled ShRNA LibraryGenome-wide Lentiviral PoolTRC CollectionHuman Genes

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